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Mass propagation of the Japanese angelica tree (Aralia elata seem.)‘Zaoh’ line No.2 was established through somatic embryos. Petioles of leaflets were cultured for induction of calli on an MS medium containing 1mgl-1 of 2, 4-D in combination with 0.5mgl-1 BA. The initiated calli were moved onto MS medium supplemented with glutamine 450mgl-1 and asparagine 300mgl-1. An embryogenic callus developed after 5months and was moved onto regulator-free MS medium. Numerous plantlets were regenerated from this embryogenetic callus, and rooted plantlets were potted after acclimation and planted to the field. The plants had the same characteristics in morphology in the field, except for the number of thorns per internode. The plants also had the same resistance to Phytophthora disease as was in the original genotype ‘Zaoh’ line No.2. This study provided a method for mass propagation from petioles of leaflets and proves that regenerated plants maintain the morphogenetic characteristics and disease resistance of the original genotype.

Somatic Embryo Formation and Plant Regeneration in 'Zaoh' line No.2 of Japanese Angelica Tree (Aralia elata seem.).