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Sedum drymarioides, an endangered plant, was micro-propagated, and the regenerated plants showed the CO2 exchange pattern of a C3-plant. Stem and leaf explants were cultured on solid MS medium containing various concentrations of 1-naphtaleneacetic acid (NAA) and 6-benzylaminopurine (BAP), resulting in callus formation at 0.1-1mgl-1 NAA. From the stem callus, the best differentiation of root and bud was observed at 1mgl-1 of NAA plus 0.1mgl-1 of BAP and 0.1mgl-1 of NAA plus 1 mgl-1 of BAP, respectively. From the leaf callus, the differentiation was most efficient with the combination of 1mgl-1 of NAA plus 1mgl-1 of BAP and 1mgl-1 of NAA plus 10mgl-1 of BAP, respectively. The buds further multiplied on medium containing 0.1mgl-1 of NAA and 10mgl-1 of BAP. They developed into rosette plantlets and grew to normal size on phytohormone-free MS medium in glass bottles, where they bolted and flowered. The regenerated plantlets were successfully transferred to pots where fertile seeds were obtained.

Reproduction of Sedum drymarioides, an Endangered Rare Species, by Micropropagation.