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We studied the effects of various treatments given before or at inoculation with Agrobacterium tumefaciens strain EHA105 (pIG121Hm) on the transient GUS gene expression in leaf segments of Saintpaulia ionantha Wendl. Sonication and vacuum infiltration treatments in the presence of Agrobacterium had no positive effect on GUS expression. In contrast, the explants cultured for 3 to 5 weeks on shoot induction medium containing 0.5mgl-1 NAA and 0.5mgl-1 BA, prior to Agrobacterium inoculation markedly increased transient GUS expression. The addition of acetosyringone to the co-cultivation medium enhanced the GUS expression. A similar enhancing effect was observed in all of the 4 cultivars examined, suggesting the wide applicability of the pre-culture treatment.

plant biotechnology

Pre-Culture Treatment Enhances Transient GUS Gene Expression in Leaf Segment of Saintpaulia ionantha Wendl. after Inoculation with Agrobacterium tumefaciens.