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Somatic embryogenesis in Ryukyumatsu (Pinus luchuensis Mayr.), an endemic species in Ryukyu Island, Japan, was initiated from megagametophytes containing zygotic embryos on a medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid and 5 μM 6-benzylaminopurine. Embryogenic cultures were maintained and proliferated by subcultures at 2to 3-week-intervals on the same fresh medium. e maturation of somatic embryos occurred on media containing maltose, activated charcoal, abscisic acid and polyethylene glycol. High frequencies of germination were obtained a er the post-maturation treatment of somatic embryos under conditions of high relative humidity, and around 90% of them were converted into plants. Growth of the somatic embryo-derived regenerated plants has been monitored in the eld.

Plant regeneration from embryogenic tissue of Pinus luchuensis Mayr, an endemic species in Ryukyu Island, Japan