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Meristems (length 100, 200, 300 µm) were excised from infected Potato plants of Binella and Burren cultivars an d cultured on solidied MS medium containing 30 g l 1 sucrose, 5 mg l 1 Ascorbic acid, 5 mg l 1 Pyridoxine, 5 mg l 1 Nicotinic acid, 5 mg l 1 iamine, 200 mg l 1 Inositole, 2 mg l 1 GA3, and 0.2 mg l 1 kinetin. Virus status of in vitro plantlets was determined by double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). Results showed that the highest rate of virus-free pl ants was obtained by using explants 100 µm in length. e rate of PVY elimination was improved aer treatment by thermotherapy at approximately 37±1°C for 40 days, (81%) in Binella and (75%) in Burren. Chemotherapy was undertaken with (10-20-30) mg l 1 of ribavirin (RBV), e highest percentage of virus free plantlets 87% in Binella and 82% in Burren were obtained from the ribavirin's concentration (20 mg l 1 ) combined with meristem- tips (100 µm) in length. Severe growth abnormalities were observed on medium containing high concentration of RBV (30 mg l 1 ). Finally, the highest percentage virus free plantlets of PVY (93% in Binella and 87% in Burren) were obtained from meristem-tips 100 µm in length excised aer electric treatments (15 mA/10 min).

Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants