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Objective: In this study, it was aimed to evaluate the efficacy of chromogenic agar for rapid and accurate identification of bacteria producing extended spectrum beta-lactamase (ESBL) and to investigate the antibiotic resistance rates of 105 bacteria that were determined to produce ESBL. Methods: ESBL production was investigated using combined disc method, E-test and chromogenic agar. Additionally, susceptibility patterns of these strains to 21 antibiotics were studied according to the criteria of CLSI (Clinical and Laboratory Standards Institute). Zone diameters categorized as susceptible were evaluated, and strains showing intermediate susceptibility were considered as resistant. Fisher’s chi-square test was used in statistical analysis. Results: A hundred and five strains (81 Escherichia coli, 24 Klebsiella spp.) were found to produce ESBL by combined disc method, while 96 strains were found to produce ESBL by E-test and 99 strains by chromogenic agar. The sensitivity and positive predictive value of chromogenic agar for ESBL production was 94.8% and 91.9%, respectively. All strains were found to be resistant to cefuroxime, cefazolin and cefotaxime, the antibiotics that ESBL producing strains are considered to be resistant to. Among beta-lactam/beta-lactamase inhibitor combinations, the highest resistance was ÖZET Amaç: Çalışmada genişlemiş spektrumlu betalaktamaz (GSBL) üreten bakterilerin doğru ve hızlı tanımlanması için kromojenik agarın etkinliğini test etmek ve GSBL ürettiği tespit edilen 105 bakteride antibiyotik direnç oranlarının tespit edilmesi amaçlanmıştır. Yöntemler: Kombine disk yöntemi, E test yöntemi ve kromojenik agar ile GSBL üretimi tespit edilmiştir. Ayrıca bu şuşların 21 antibiyotiğe karşı duyarlılıkları CLSI (Clinical Laboratory Standards Institute) kriterlerine göre çalışılmıştır. Duyarlı kabul edilen zon çapları değerlendirilmiş, orta duyarlı suşlar dirençli kabul edilmiştir. İstatistiksel değerlendirme Fisher’s ki-kare testiyle yapılmıştır. Bulgular: Kombine disk yöntemi ile 105 (81 tanesi Escherichia coli ve 24 tanesi Klebsiella spp.), E test ile 96 ve kromojenik agar ile 99 suşun GSBL ürettiği tespit edilmiştir. Kromojenik agar yöntemi ile GSBL tespitinin duyarlılığı %94,8. GSBL üreten suşların dirençli kabul edildiği sefuroksim, sefazolin ve sefotaksim’e tüm suşların dirençli olduğu görülmüştür. Beta-laktam/ beta-laktamaz inhibitörü kombinasyonları içerisinde ampisilin-sulbaktam direncinin (%75,2) yüksek olduğu bu grupta en az dirençli antibiyotiklerin piperasilintazobaktam (%31,4) ve sefoperazon -sulbaktam (%32,4) olduğu tespit edilmiştir. Karbapenemlere (imipenem, 1 Univercity of Sofia “st. Kliment Ohridski” Faculy of Biology, Department of Mikrobiology, SOFIA, BULGARIA 2 Ankara Numune Research and Education Hospital, Medical Mikrobiology, ANKARA, TURKEY Geliş Tarihi / Received : Kabul Tarihi / Accepted : İletişim / Corresponding Author : Mustafa GÜZEL Univercity of Sofia “st. Kliment Ohridski” Faculy of Biology, Department of Mikrobiology, SOFIA, BULGARIA Tel : +90 532 494 32 50 E-posta / E-mail : dr.mustafaguzel@hotmail.com DOI ID : 10.5505/TurkHijyen.2015.33239 Türk Hijyen ve Deneysel Biyoloji Dergisi Güzel M, Genç Y, Aksoy A, Moncheva P, Hristova P. Comparison of three different methods for detection of ESBL production and antibiotic resistance percentage of ESBL producing Gram negative bacteria. Turk Hij Den Biyol Derg, 2015; 72(2): 131-8. 20.03.2015 05.06.2015 Makale Dili “İngilizce”/Article Language “English”

türk hijyen ve deneysel biyoloji dergisi/türk hijiyen ve deneysel biyoloji dergisi

Investigation of three different methods for detection of ESBL production and antibiotic resistance percentage of ESBL producing Gram negative bacteria