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Objective: The aim of this study was to evaluate the microbial contamination and bacterial endotoxin levels in dialysis water samples sent to the Water and Food Microbiology Laboratory of the Adana Hygiene Institute Refik Saydam Hygiene Center, and to assess factors that might influence the results of these tests. Method: Two hundred fourty five dialysis water samples sent to the laboratory between January 2009 and December 2010 were tested; all of them for microbial contamination and 198 of them for bacterial endotoxin levels. Microbial contamination was evaluated by using the Plate Count Agar (PCA) method while the Limulus Amoebocyte Lysate (LAL) assay was used for the detection of bacterial endotoxins. The results were evaluated according to the criteria of the “Directives on the Water Purification Systems” and “the European Pharmacopoeia”. To compare the annual differences, the chi-square test was used for statistical evaluation and results OZET Amac: Bu calismada Refik Saydam Hifzissihha Merkezi Baskanligi Adana Hifzissihha Enstitusu Mudurlugu Su ve Gida Mikrobiyoloji Laboratuvarina kontrol amaciyla gelen diyaliz sularinin mikrobiyal kontaminasyon ve bakteriyel endotoksin test sonuclari ile bu testlerin birbiriyle olan iliskilerinin degerlendirilmesi amaclanmistir. Yontem: Bu calismada Ocak 2009Aralik 2010 tarihleri arasinda laboratuvarimiza gelen 245 adet diyaliz suyu incelenmistir. Mikrobiyal kontaminasyon orneklerin tamaminda, bakteriyel endotoksin ise sadece 198 ornekte calisilmistir. Mikrobiyal kontaminasyon calismalari, Plate Count Agar (PCA) ile yapilmistir. Bakteriyel endotoksinin tespiti icin ise Limulus Amoebocyte Lysate (LAL) yontemi calisilmistir. Mikrobiyal kontaminasyon ve bakteriyel endotoksin testlerinden elde edilen sonuclar “Su Aritma Sistemi Yonergesi” ve “Avrupa Farmokopesi”nde belirtilen kriterlere gore degerlendirilmistir. Sonuclarin yillara gore degisimini karsilastirmak icin istatistiksel degerlendirmelerde ki-kare testi kullanilmis ve 1 Adana Hifzissihha Enstitusu Mudurlugu, ADANA Gelis Tarihi / Received : Kabul Tarihi / Accepted : Iletisim / Corresponding Author : Fesem BAŞARI Adana Hifzissihha Enstitusu Mudurlugu, ADANA Tel : +90 322 453 33 41 E-posta / E-mail : fesemb@yahoo.com 06.06.2011 15.02.2012 DOI ID : 10.5505/TurkHijyen.2012.47135 Turk Hijyen ve Deneysel Biyoloji Dergisi Basari F, Uyanik O. Diyaliz sularinin mikrobiyal kontaminasyon ve bakteriyel endotoksin testleri ile kontrolu. Turk Hij Den Biyol Derg, 2012; 69(1): 7-14. Cilt 69  Sayi 1  2012 Turk Hij Den Biyol Derg 8 Bobrek yetmezligi sikâyeti olan hastalar normal populasyonla karsilastirildiginda yasamlari suresince daha fazla miktarlarda suya maruz kalmaktadirlar. Bobrek yetmezligi olan hastalara diyaliz sirasinda her hafta yaklasik 360-400 litre su uygulanirken, saglikli bir insan her hafta yalnizca 14-15 litre su almaktadir. Bu hesaplamalara gore diyaliz hastalari yillik olarak 18.000 -36.000 litre arasinda suya maruz kalmaktadir. GIRIŞ DIYALIZ SUYU, MIKROBIYAL KONTAMINASYON, ENDOTOKSIN p<0,05 olanlar istatistiksel olarak anlamli kabul

Control of Dialysis Waters by Microbial Contamination and Bacterial Endotoxin tests