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Objective: The aim of this study was to contribute to the antibiotic usage policies by determining microorganisms isolated from blood cultures at Microbiology Laboratory of 82. Year Rize Governmental Hospital between February 2010 and February 2011, and their sensitivity to antibiotics. Method: Nine-hundred blood culture samples sent to the laboratory were added to biphasic blood culture medium (Rosmedia, GBL) and were inoculated both with EMB and sheep blood agar according to the routine procedures. The identification of microorganisms were made with conventional methods including colony morphology, Gram staining and biochemical tests. Antibiotic susceptibility was determined by Kirby-Bauer disk diffusion method according to the recommendations of Clinical and Laboratory Standards Institute (CLSI). Results: There was no growth in 750 (83.3%) of these samples and 15 (1.7%) samples were concidered as contaminations. Bacteria were determined in 135 (15.0%) samples; 108 (80%) of these were Grampositive, 23 (17.0%) were Gram-negative and 4 (3.0%) were Candida spp.. In the group of Gram-negative bacteria, coagulase negative Staphylococcus (CNS) was the most frequently isolated species 94 (87%), followed by Staphylococcus aureus with 4 (3.7%) and OZET Amac: Bu calismada Şubat 2010 Şubat 2011 tarihleri arasinda Rize 82. Yil Devlet Hastanesi Mikrobiyoloji Laborotuvarina gonderilen kan kultur orneklerinden izole edilen cesitli mikroorganizmalar ve antibiyotik duyarliliklari arastirilmis ve uygun antibiyotik kullanim politikalarina katkida bulunulmasi amaclanmistir. Yontem: Laboratuvarimiza gonderilen 900 kan kulturu ornegi bifazik Rosmedia (GBL), besiyerine ekilmis ve rutin prosedurlere uygun olarak EMB ve koyun kanli agara pasajlanmistir. Ureme sonrasi mikroorganizmalarin identifikasyonu koloni morfolojisi, Gram boyama ve biyokimyasal testleri iceren konvansiyonel yontemlerle yapilmistir. Antibiyotik duyarliliklari Clinical and Laboratory Standards Institute (CLSI) onerilerine gore KirbyBauer disk difuzyon yontemi ile belirlenmistir. Bulgular: Gelen kan kultur orneklerinin 750 (%83,3)’sinde herhangi bir ureme olmamis, 15 ornek (%1,7) ise kontaminasyon olarak degerlendirilmistir. Orneklerin 135 (%15.0)’inde cesitli mikroorganizmalar izole edilmis, 108 (%80,0)’i Gram-pozitif bakteriler, 23 (%17,0)’u Gram-negatif bakteriler ve dordu (%3,0) Candida spp. olarak bulunmustur. Gram-pozitif bakterilerden 94 (%87,0)’u koagulaz-negatif stafilokok (KNS), dort (%3,7)’u S.aureus ve 10 (%9.3)’u Enterococcus 1 Rize Universitesi Tip Fakultesi, Tibbi Mikrobiyoloji Anabilim Dali, RIZE 2 Rize 82. Yil Devlet Hastanesi, Klinik Mikrobiyoloji Laboratuvari, RIZE 3 Rize Universitesi Tip Fakultesi, Enfeksiyon Hastaliklari ve Klinik Mikrobiyoloji Ana Bilim Dali, RIZE 4 Rize 82. Yil Devlet Hastanesi, Anesteziyoloji ve Reanimasyon Klinigi, RIZE Gelis Tarihi / Received : Kabul Tarihi / Accepted : Iletisim / Corresponding Author : Aysegul COPUR-CICEK Rize Universitesi, Tip Fakultesi, Tibbi Mikrobiyoloji Anabilim Dali, RIZE Tel : +90 464 212 30 09 E-posta / E-mail : draysegulcicek@yahoo.com 05.07.2011 22.11.2011 DOI ID : 10.5505/TurkHijyen.2011.66588 Turk Hijyen ve Deneysel Biyoloji Dergisi * Bu calisma 18-22 Mayis 2011 tarihinde 26.ANKEM (Antibiyotik ve Kemoterapi) Kongresinde poster (P40) olarak sunulmustur. Copur-Cicek A, Şenturk-Koksal Z, Erturk A, Koksal E. Rize 82. Yil Devlet Hastanesi’nde bir yillik surede kan kulturlerinden izole edilen mikroorganizmalar ve antibiyotiklere duyarliliklari. Turk Hij Den Biyol Derg, 2011; 68(4): 175-84. Cilt 68  Sayi 4  2011 Turk Hij Den Biyol Derg 176 Dolasim sistemi enfeksiyonlari antimikrobiyal ve destekleyici tedavilere ragmen morbidite ve mortalitenin major nedenleri olmaya devam etmektedir. Bu nedenle kan dolasimi enfeksiyonlarinin erken tanisi ve uygun tedavi edilmesi klinik acidan onemlidir. Kan kulturleri supheli enfeksiyon vakalarinda mikrobiyal etyolojiyi tanimladigi gibi, tedavinin yonlendirilmesinde de rol oynar (1,2) Bakteriyemilerde etken mikroorganizmalarin dagilimlari ve antibiyotik duyarliliklari yillara gore degisiklikler gostermektedir. Ampirik tedavide yol gostermesi acisindan etken mikroorganizma ve antibiyotik duyarliliklarinda olusan degisiklikler her merkez tarafindan surekli olarak belirlenmelidir (3,4). Bu calismada cesitli kliniklerden gonderilen kan kulturlerinde ureyen mikroorganizmalarin antimikrobiklere karsi duyarliliklari arastirilarak antibiyotik kullanim politikalarina katkida bulunulmasi amaclanmistir.

Microorganisms isolated from blood cultures during the period of one year at the 82nd Year Rize State Hospital and their susceptibility to antibiotics