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.Immunophenotyping was performed using CD45, CD19, CD3, CD5, CD7, CD4, CD8, CD38, CD56, kappa, and lambda light chains. gating was performed using for-ward-side scatter and CD45-side scatter.A single large cluster observed in the lymphocyte region of the SSC/CD45 side scatter showed a high level of CD45 expression and was composed predominantly of T-cells (CD3+) with moderately high CD38 positivity (T-cell activation marker). nothing abnormal with respect to T-cell markers (CD3/CD5/CD7) was noted. reversal of the CD4:CD8 ratio (0.1) was also noted. CD19, kappa, lambda, and CD56 expression was not observed. Immu-nophenotyping results suggested non-neoplastic prolifera-tion of lymphocytes—primarily involving a T-cell compo-nent with an inverted CD4:CD8 ratio, which could also be a manifestation of immunosuppressive states and viral infections, e.g. EBv, Cmv, and HIv [1]. EBv Igm was positive (17.58 U ml

Flow cytometry - A rapid & robust adjuvant technique for pathological diagnosis