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Adsorption of fibrinogen on positively charged microspheres was theoreticallyand experimentally studied. The structure of monolayers and the maximumcoverage were determined by applying the experimental measurements at pH=3.5and 9.7 for NaCl concentration in the range of $10^{-3}- 0.15$ M. The maximumcoverage of fibrinogen on latex particles was precisely determined by the AFMmethod. Unexpectedly, at pH=3.5, where both fibrinogen molecule and the latexparticles were positively charged, the maximum coverage varied between 0.9 mgm$^{-2}$ and 1.1 mg m$^{-2}$ for $10^{-2}$ and 0.15 M NaCl, respectively. Onthe other hand, at pH=9.7, the maximum coverage of fibrinogen was larger,varying between 1.8 mg m$^{-2}$ and 3.4 mg m$^{-2}$ for $10^{-2}$ and 0.15 MNaCl, respectively. The experimental results were quantitatively interpreted bythe numerical simulations.

Modelling and measurements of fibrinogen adsorption on positively charged microspheres