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To identify gene whose expression is enhanced by low level laser irradiation (LLLI ; 830 nm Ga-Al-As diode laser), we constructed the cDNA library of osteoblastic cell line MC3T3-E1 using a subtractive gene cloning. In the present study, we focused on a gene clone designated as MCL98. DNA sequence of MCL98 indicated the high homology with inhibin beta (INHBB) gene. The enhancement of INHBB mRNA by LLLI was successfully confirmed by reverse transcription-polymerase chain reaction and real-time PCR. INHBB is shown to serve as a co-receptor for BMPs which stimulate bone cell differentiation and bone formation. Thus, the enhancement of INHBB expression by LLLI may be one of molecular mechanisms in accelerating bone formation by LLLI.

Identification of Inhibin B Gene Expression in Osteoblast by Low Level Laser Irradiation