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Since the discoveries of specific oligosaccharide forming amylases, such as Exo-maltotetrao hydrolase, Exo-maltohexaohydrolase etc., a large scale preparation of maltooligosaccharides become feasible. The several possible methods to prepare maltooligosaccharides are discussed in this paper as well as the fractionation method such as charcoal column chromatography, gel filtration chromatography. Quantitative and qualitative analyses of various oligosaccharides are discussed with some examples of the analysis and characteristics of each method.

Preparation, Fractionation and Analysis of Maltooligosaccharides