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Three crystal structures of endopolygalacturonase I (EndoPG I) from Stereum purpureum have been determined in this study: the unliganded EndoPG I, the binary and ternary complexes of EndoPG I with galacturonate. Consequently, the structural basis for substrate binding and the catalytic mechanism of EndoPG I have been elucidated by X-ray crystallography. Crystals of deglycosylated EndoPG Ia have been obtained using PEG4000 as precipitate with the hanging-drop vapor diffusion method. The crystal belongs to space group P 1, with unit-cell parameters a=37.26 A°, b=46.34 A°, c=52.05 A°, α=67.17°, =72.44°, γ=68.90°. The crystal diffracts to ultra-high (0.96 A°) resolution using synchrotron radiation of SPring-8. Crystal structures of EndoPG I were determined by the multiple wavelength anomalous dispersion (MAD) method. For MAD phasing, three wavelength data sets of K2PtCl4 derivative crystal were collected at SPring-8. The structure model was refined anisotropically with SHELXL-97, with an R factor of 11.4% and an Rfree factor of 14.0% at 0.96 A°resolution. The enzyme folds into a right-handed parallel -helix with 10 complete turns. The crystal structures of its binary complex with one D-galacturonate and its ternary complex with two Dgalacturonates were also determined to identify the substrate binding site at 1.0 and 1.15 A°resolutions, respectively. In the binary complex, one -D-galactopyranuronate, GalpA, was found in the reducing end side of Asp153, Asp173 and Asp174, which are considered as candidates of catalytic residues. This reveals that the position of GalpA is the +1 subsite, thus proving the strong affinity of the +1 subsite expected from the bond cleavage frequency on oligo-galacturonates. In the ternary complex, an additional -Dgalactofuranuronate was found in the -1 subsite. In both subsites, the recognition of the galacturonate carboxy group is important in galacturonate binding. In the +1 subsite, the carboxy group interacts with three basic residues, His195, Arg226 and Lys228, which were conserved in all endopolygalacturonases. In the -1 subsite, the unique non-prolyl cis-peptide bond is believed to be involved in binding the carboxy group of the substrate. Based on the structures of Galf A and GalpA bound in the ternary complex, a structural model of the di-galacturonic acid part of the substrate molecule bound in both the -1 and +1 subsites across from the catalytic residues was constructed. The di-galacturonate model structure sheds light on the catalytic mechanism. Asp173 is at the appropriate position to be a proton donor to the fissile glycosidic bond. Asp153 or Asp174 seems to act as a general base to abstract a proton from the nucleophilic water.

Reaction Mechanism Based on X-ray Crystallography at Atomic Resolution of Endopolygalacutronase I from Fungus <i>Stereum purpureum</i>