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The aim of this study was to evaluate and compare the efficacy of different CIDR protocols including some hormonal preparations for the treatment of postpartum anestrous of Iraqi northern buffaloes. The present study was conducted on 70 anestrous buffaloes suffering from postpartum ovarian inactivity. Buffaloes were randomly assigned to three treatment groups. Buffaloes in Treatment 1 (n=20) injected on day 0 of the experiment, GnRH 250 ig and a CIDR was placed in the anterior vagina. On day 7, they were injected with 25 mg of PGF2a and on the following day (day 8) the CIDR were removed. On day 9 a second dose of GnRH (250 ig) was injected. Buffaloes in Treatment 2 (n=20) received on day 0 GnRH (250 ig) and a CIDR were placed in the anterior vagina. On day 7, CIDR were removed and injected with GnRH (250 ig). Treatment 3 (n=20) on day 0 a CIDR were placed in the anterior vagina. On day 6, they were injected with 1000 IU of eCG. CIDR were removed on day 7 and injected with GnRH (250 ig). A control group (n=10) received no treatments. Results showed that in 8days treatment 1 (GnRH+CIDR— —7 PGF a+9 GnRH), 15 out of 20 buffaloes received treatment exhibited estrus 2 within 69.4 ± 8.4 h after CIDR removal. The estrus induction rate was 75%. Total number of buffaloes became 7 days pregnant was 4 (26.7%). In treatment 2 (GnRH+CIDR— —GnRH), 65% of the buffaloes received treatment exhibited estrus within 77.8 ± 5.6 h after CIDR removal. Total number of buffaloes became pregnant was 4 7 days (26.7%). In treatment 3 (CIDR— — 6 eCG+7 GnRH), 100% of the buffaloes received treatment exhibited estrus within 78.3 ± 11.8 h after CIDR removal. In conclusion, our results indicate that the addition of GnRH and eCG to a progesterone-based CIDR protocol substantially improves the estrus induction and pregnancy rates in postpartum anestrous buffaloes.

Comparative efficacy of different CIDR protocols for the treatment of postpartum anestrous in Iraqi buffaloes