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Diarrhoeal diseases are important cause of morbidity and mortality in neonates of various animal species. Rotaviruses cause neonatal diarrhea in calves. The present study was carried out to determine the prevalence of rotavirus infection among cow calves in Mathura and adjacent regions. During the present study 101 diarrheic and 29 non diarrheic stool samples collected from cow calves were screened for rotavirus. Of the101 diarrheic samples 17 samples (16.83%) were found to be positive for rotavirus by RNA PAGE. All the non-diarrheic samples were negative for rotavirus. All the isolates exhibited 4-2-3-2 migration pattern suggesting group A rotavirus. Depending on migration of 10th and 11th segments, all the isolates were of long pattern. Three different electropherotypes were detected in this study period. Male diarrheic calves were found to be more susceptible to rotavirus infection (20.37%) than female diarrheic calves (12.76%). Besides Rotavirus antigen was detected by ELISA.

Detection of Rotavirus from diarrhoeic cow calves in Mathura, India