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The survival and infectivity of indigenous isolates of Haemonchus contortus and in vitro cultivated infective larvae (L ) were studied. Active motile gravid females, adult and immature parasites were morphologically and 3 morphometrically characterized and isolated from Haemonchus infected goats. These were subsequently inoculated into the abomasum of parasite free lambs through left sided laparatomy for production of fertile eggs to be used for 0 coproculture. Harvested faeces from these lambs were cultured in a nutritive medium and kept in the incubator at 32 C 0 and room temperature 24-28 C to obtain in vitro cultivated L of H. contortus. The infectivity and survival of in vitro 3 0 0 cultivated L stored in refrigerator at 4-8 C and under room temperature 24-28 C were evaluated. Incubation period of 3 o 0 coproculture was observed to be shorter in medium at 32 C than under room temperature 24-28 C. The storage of in vitro cultivated L under different temperature range showed that the quantity of active larvae in a given volume 3 th 0 0 th decreased at p<0.05 from the 5 day under 24-28 C while at 4-8 Cbecame significantly lower from 20 day. Infectivity 0 0 of isolate kept at 4-8 C was sustained for 18-20 days while at 24-28 C it was maintained for 10-12 days. Laboratory invitro cultivated L of H. contortus had limited infectivity and larvae mortality increase with time under different 3 temperatures.

Survival and infectivity studies of in-vitro cultivated larvae of Haemonchus contortus in Sheep and Goats in Nigeria