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O objetivo do presente estudo foi testar protocolo hormonal para inseminação em tempo fixo (IATF) usando dispositivo de progesterona vaginal por seis dias e aplicação de eCG (gonadotrofina coriônica equina) ou BE (benzoato de estradiol) como indutor de ovulação em ovelhas. No dia 0 (D0), as ovelhas (n=31) receberam CIDR e foram divididas em três grupos. O grupo controle (G-CT) usou CIDR por nove dias e na retirada do dispositivo foi administrado PGF2a e eCG. No grupo eCG (G-eCG), CIDR foi mantido por seis dias, ao invés de nove como no G-CT, com aplicação de PGF2a e eCG na retirada da progesterona. No grupo benzoato de estradiol (G-EB), o CIDR também foi administrado por seis dias, mas não foi utilizado eCG, o BE foi usado para induzir ovulação e foi aplicado 24 horas após a remoção do CIDR e administração de PGF2a. A IATF foi feita em todos os grupos 50h após a retirada do CIDR. A taxa de prenhez do G-eCG (66%) foi maior (p <0.05) que no G-EB (11,1%). O grupo G-eCG de curta duração não diferiu (p>0,05) do G-CT (30%). É possível reduzir o tempo de permanência da progesterona usando eCG para IATF em ovelhas, entretanto o uso de BE resultou em baixa taxa de ovulação.The aim of this study was to test the hormonal protocol for fixed-time artificial insemination (TAI) with a progesterone vaginal device for six days applying equine chorionic gonadotropin (eCG) or estradiol benzoate (EB) as an ovulation inducer in ewes. On day 0 (D0), the ewes (n=31) received a device containing 0.33 g of intravaginal progesterone (CIDR®) and were divided into three groups. The G-CT (control group) used CIDR for nine days and upon withdrawal of the progesterone, were administered prostaglandin F2 alpha (PGF2?) and eCG. In the eCG (G-eCG) group, CIDR was administered for six days, instead of nine as in the G-CT group, followed by PGF2a and eCG after progesterone withdrawal. In the EB (G-EB) group, CIDR was also administered for six days, but eCG was not applied, and EB was used to induce ovulation 24 hours after removing the CIDR and PGF2a application. TAI was realized in all groups 50 hours after CIDR withdrawal. The pregnancy rate in the G-eCG group (66%) was higher (p <0.05) than that in the G-EB group (11.1%). The short-term groups showed no difference (p>0.05) compared to the G-CT group (30%). It is possible to reduce the application time of the progesterone and use eCG for TAI in sheep; however, using EB resulted in a low ovulation rate in the sheep

Use of estradiol benzoate to induce ovulation in a short-term protocol for fixed-time AI in sheep