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The aim of this study was to investigate the effect of different feeding proportions of forage ―grazing vs. silage― on milk fatty acids (FA) profile and conjugated linoleic acid (CLA) content of autumn calving Holstein-Friesian cows (n = 61) at CIAM (Galicia, NW Spain). Three treatments (S, 100% silage; G/S, 50% grazing + 50% silage; G, 100% grazing) were set and milk FA profile of dairy cows was determined by gas chromatography-mass spectrometry. The G group showed a decrease in short ( p < 0.05) and medium chain FA ( p < 0.001), with an increase in long chain FA ( p < 0.001) in comparison to the G/S and S groups, which showed the lowest levels ( p < 0.001) of mono- and polyunsaturated FA. The CLA content in milk fat increased ( p < 0.001) linearly in relation to the increased proportion of fresh grass in the diet of dairy cows from 0.49 and 0.82 to 1.14 g/100 g FA for the treatments S, G/S and G, respectively. During spring and summer, the levels of CLA were three times higher ( p < 0.001, +0.76 g/100 g FA) in milk from dairy cows at the G group than in cows at the S group and twice higher ( p < 0.001, +0.40 g/100 g FA) than in cows at the G/S group. High proportion of grass in the diet of cows increased CLA content, with the highest levels of unsaturated FA and the lowest levels of saturated FA, increasing the added value of milk on grazing systems using available farm resources.

spanish journal of agricultural research

Short communication. Effect of forage source (grazing vs. silage) on conjugated linoleic acid content in milk fat of Holstein-Friesian dairy cows from Galicia (NW Spain)