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Inoculation of Apis mellifera colonies with chalkbrood is a good way to test for hygienic behavior. The use of compatible pure strains of Ascosphaera apis, the causative agent of chalkbrood, could maintain homogeneity among experiments. Mycelium aging, viability, purity and capacity to produce spore-cysts were evaluated in MY20 and integral rice kernels (IRK) medium. Ascosphaera apis strains on MY20 developed aging symptoms by the 30th day and developed pure colonies until the 77th day. The same strains on IRK showed aging symptoms by the 30th day of growing, but they produced pure colonies for 360 days. Spore-cysts obtained from pure strains preserved in IRK and spore-cysts from wild black mummies obtained from honeybee hives were used to inoculate fifth instar A. mellifera larvae. Ascospores from black wild mummies or spore-cysts obtained from pure strains preserved in IRK were equally effective in causing clinical symptoms of the disease, which has main advantages: permanent inoculum availability and genetic homogeneity.

Short communication. Viability and pathogenicity of Ascosphaera apis preserved in integral rice cultures