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Cryopreservation is the conservation at very low temperature (</= 150 deg C) of living propagules. Cryopreservation offers a viable and economical method for the long-term conservation of genetic resources of vegetatively propagated plants. Various techniques have been developed to minimize desiccation and freezing damage, thus ensuring high propagule recovery. In most cases, shoot apices obtained from in vitro-grown shoots are the plant material used for cryopreservation of vegetatively propagated plants. Cryopreservation techniques are based on either freeze-induced dehydration ("classical methods"), or vitrification of internal solutions ("new methods"). Various considerations should be taken into account when cryopreservation techniques are actually used for germplasm conservation.

Review. The use of cryopreservation for germplasm conservation of vegetatively propagated crops