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consumed as a vegetable and as a source of herbal medicines in East Asia. In Korea, two virus species including Broad bean wilt virus 2 (BBWV2), and Chinese yam necrotic mosaic virus (CYNMV) have been reported to infect Chinese yam (Kang et al., 2003; Kwon et al., 2016). Infection of Chinese yam with CYNMV was the first report in Japan in 1978 (Fukumoto and Tochihara, 1978). Since then, CYNMV has been considered of important virus in Chinese yam because of serious yield loss (Kondo, 2001a; Kondo and Fujita, 2012; Kondo et al., 2007). While BBWV2 infection of Chinese yam was identified very recently in Korea, the infection of Chinese yam with CYNMV was reported in 2003 in Korea (Kang et al., 2003). CYNMV, a member of the genus Macluravirus in the Family Potyviridae, is a flexuous, filamentous virus with 660 nm in length (Fukumoto and Tochihara, 1978). CYNMV is easily trans­mitted by aphids in a nonpersistent manner but its host range is restricted to Dioscorea spp. (Fukumoto and Tochihara, 1978; Kondo et al., 2015). The size of the complete genome of CYNMV is approximately 8,230 nucleotides (nt). Currently, only two complete genome sequences of the CYNMV isolates (one from Japan and another from China) are available in the GenBank database. A previous study reported the 3’-proximal partial sequences of a CYNMV Korean isolate but no complete genome sequence of CYNMV isolated in Korea has been determined yet (Kondo et al., 2003). Therefore, we decided to determine the complete genome sequence of a CYNMV Korean isolate to examine molecular characteristics of the Korean isolate and to generate molecular detection primers specific for CYNMV Korean isolates.

singmulbyeong yeon-gu

Complete Sequence Analysis of a Korean Isolate of Chinese Yam Necrotic Mosaic Virus and Generation of the Virus Specific Primers for Molecular Detection