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Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging\uddiseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and\udaccurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae\udDNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In\udsensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml\udof genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time\udPCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific\udprimer set could be useful for rapid and accurate detection of P. brassicae

Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage