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A rapid, user-friendly and simple immune-chromatographic dipstick kit named ‘rapid immune-gold strip’ (RIGS)\udkit was developed in a novel single strip format to detect on-site detection of Tomato spotted wilt virus (TSWV).\udImmunoglobulin G (IgG) from polyclonal antisera raised in rabbits against TSWV was purified through protein-\udA affinity chromatography and then the purified TSWV-IgG was conjugated to colloidal gold nano-particles\udwhich served as a test line on nitrocellulose membrane. Protein A that non-specifically binds to TSWV antibody\udwas used as a control line on the same strip. The diagnosis process with the TSWV-RIGS involves simply\udgrinding the suspect plant sample in a bag that contains the extraction buffer and inserting the strip the bag.\udResults can be seen in 2-5 minutes. The flow of the complexes of gold particles coated with TSWV-IgG and a\udcrude sap from TSWV-infected pepper, tobacco and tomato plants resulted in intensive color formed on the\udtest lines proportional to the concentrations of TSWV. The RIGS-TSWV kit did not show any cross-reactions\udagainst other tomato-infecting viruses unrelated to TSWV. These results indicate that the TSWV-RIGS kit is\udhighly sensitive and is not required for laboratory training and experience prior to testing. The TSWV-RIGS kit\udis suitable for on-site detection of suspect TSWV-infected plants as well as for laboratory diagnosis

Development of Rapid Immune-gold Strip Kit for On-Site Diagnosis of Tomato spotted wilt virus