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C. Among the nine agro-chemicals, oxolinic acid WP,streptomycin + copper hydroxide WP and streptomycin WP were found to be effective for the inhibition ofthe pathogen in vitro. The results of scanning electron microscopic investigation showed that onion bulbs wasmacerated by infection of B. gladioli pv. alliicola CH1. B. gladioli pv. alliicola CH1 was able to producepolygalacturonase but did not produce pectin lyase and carboxymethylcellulase. In analysis of thepolygalacturonase activity of the isolated pectin-degradation enzymes from B. gladioli pv. alliicola CH1 totalprotein, three activity bands 45 kDa, 35 kDa, and 29 kDa were detected by the direct (or in-gel) activitystaining on SDS-PAGE. Keywords : Burkholderia gladioli pv. alliicola, Polygaractronase, SDS-PAGE(pectin)  1%     1               .            ,          .             .    cellulase,pectinase, polygalacturonase(PGase), protease (Fukumori, 1988)     ,                (Collmer, 1986; Daniels , 1988).     Pseudomonasgladioli pv. alliicola(Burkholder, 1942), Pseudomonascepacia(Burkholder, 1950), Pseudomonas aeruginosa (Cother, 1976), Erwinia carotovora subsp. carotovora (1901)

Phytopathogenicity of Burkholderia gladioli pv. alliicola CH1 and Production of PGase Isozymes