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Rice bakanae disease, caused by the fungus Fusarium fujikuroi, is one of the most important rice diseases and distributed widely in Asia. Resistance screening system in rice field had been established. However, the evaluation results of the system vary according to the environmental conditions when the test is conducted. To develop precise and rapid evaluation method of disease resistance of rice to bakanae disease, in vitro screening system was attempted in this study. The six cultivars namely, `Nampyeongbyeo`, `Junambyeo`, `Chucheongbyeo`, `Samcheonbyeo`, `Odaebyeo` and `Hwasinbyeo` were tested. They were planted onto MS agar medium (10 ml) in test tube () and incubated at and in growth chamber under 12 hr light condition. Symptoms of over growth appeared a few days after seeding and then seedling were withered 2-3 weeks after over growth. The disease symptoms such as leaf dryness on top of rice were appeared in the `Nampyeongbyeo` from 28 days at the concentration of spores/ml culturing at and then withered completely 35 days after seeding. Whereas the other varieties withered entirely 19-23 days after seeding. Using the in vitro seedling screening method, 72 rice varieties were investigated to select resistant cultivar. Finally, two resistant cultivars (`Nampyeongbyeo` and `Inwolbyeo`) and seven moderately resistant cultivars (`Hwadongbyeo`, `Seokjeongbyeo`, `Samgwangbyeo`, `Sampyeongbyeo`, `Nonghobyeo`, `Heukjinjubyeo` and, `Joanbyeo`) were selected. If in vitro seedling screening method was used for evaluation of bakanae disease resistance, it would be completed within 35 days after sowing of rice seed.

Development of In Vitro Seedling Screening Method for Selection of Resistant Rice Against Bakanae Disease