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4 ABSTRACT Considering that the MS medium is very rich in mineral salts ally to the high cost and the difficulty in the acquisition of nitrate and ammonium it was aimed at to reduce the concentration of two sources of nitrogen (NH4NO3 and KNO3) used in the culture medium of nodal segments of Coffea arabica L. cv. Rubi. Explants (2 cm) preestablished in vitro were inoculated in MS medium with NH4NO3 and KNO3 (0, 25, 50, 75 and 100 %) and maintained at growth room with 27±1 oC temperature and 16 hours/day under 32 mM.m -2 .s -1 light intensity. After 60 days were evaluated the total number of sprouts, the aerial part fresh matter weight and the total dry matter weight. Its possible to reduce the NH4NO3 concentration to 75% and KNO 3 to 50%.

FONTES DE NITROGÊNIO NA MICROPROPAGAÇÃO DE Coffea arabica