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Uma linhagem de Acinetobacter baumanii, isolada da rizosfera de cana-de-acucar, cultivada em solos tratados com Diuron foi incubadada em diferentes substratos para verificar a presenca de catecol 1,2- dioxigenase (pirocatecase). Diferentes indutores foram adicionados ao meio de cultura (glicose, benzoato de sodio, Diuron, dicloroanilina, benzoato de sodio + glicose, benzoato de sodio + dicloroanilina, Diuron + glicose). As celulas bacterianas, obtidas pelo processo fermentativo, foram coletadas apos centrifugacao e rompidas por sonicacao para extracao da enzima intracelular. A linhagem apresentou alta atividade enzimatica quando benzoato de sodio ou benzoato de sodio + glicose ou benzoato de sodio + dicloroanilina foram adicionados ao meio de cultura. A atividade enzimatica acompanhou a producao de biomasa. No estudo da cinetica de crescimento, usando Diuron como fonte de carbono e em tres diferentes pH, essa linhagem apresentou melhor resultado quando cultivada em pH 6,8. Verificou-se pela avaliacao da capacidade de A. baumanii transformar o Diuron, que o metabolito 3,4-dicloroanilina (3,4-DCA) nao foi produzido. PYROCATECHASE INDUCED BY Acinetobacter baumanii INVOLVED IN THE BIODEGRADATION OF THE HERBICIDE DIURON Abstract An Acinetobacter baumanii strain, isolated from Diuron treated sugarcane rhizosphere, was grown in different substrates in order to determine the catechol 1,2-dioxygenase (pyrocatechase) presence. Different inducers were added to the culture media (glucose, sodium benzoate, Diuron, dichloroaniline, sodium benzoate plus glucose, sodium benzoate plus dichloroaniline, and Diuron plus glucose). The bacterial cells were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. The strain showed high activity of pyrocatechase when sodium benzoate or sodium benzoate plus glucose or sodium benzoate plus dicloroaniline were added to the culture media. The enzymatic activity followed the biomass production. According to the growing kinetics study using Diuron as a carbon source, and with three different pH, this strain showed the best result when growed at pH 6.8. Estimating the A. baumanii capacity of transforming Diuron, it was observed that the 3,4-dichloroaniline (3,4-DCA) metabolite was not produced.

INDUÇÃO DA ENZIMA PIROCATECASE POR Acinetobacter baumanii ENVOLVIDA NA BIODEGRADAÇÃO DO HERBICIDA DIURON