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A citologia aspirativa com agulha fina (CAAF) foi usada como metodo de colheita de quinze amostras de tumor venereo transmissivel (TVT)em caes, oriundos do atendimento do Hospital Veterinario-FMVZ-UNESP de Botucatu, antes e apos o inicio da quimioterapia. As amostras citologicas foram submetidas ao calculo do indice mitotico e a avaliacao de indices de proliferacao por metodos citoquimico (concentracao eletrolitica critica  CEC) e imunocitoquimico (Ki-67, clone MIB-1). Todas as tecnicas mostraram-se adequadas para avaliar a taxa proliferativa em amostras citologicas. O CEC permitiu observar que a aplicacao do quimioterapico levou a uma reducao significativa da proliferacao celular. Em vista disso, conclui-se que essa metodologia pode ser aplicada para diagnosticar e monitorar o TVT. Proliferation indexes determination by CEC and Ki-67 in fine needle aspiration cytology of transmissible venereal tumor Abstract Fine needle aspiration biopsy (FNAB) was used to collect fifteen transmissible venereal tumor (TVT) samples from dogs at the Veterinary Hospital - FMVZ - UNESP Botucatu, Sao Paulo, Brazil, before and after beginning of chemotherapy. The cytological samples were submitted to determination of mitotic and proliferation indexes by means of cytochemical (critical electrolyte concentration  CEC) and immunocytochemical (Ki-67, MIB-1 clone) methods. All the techniques were adequate for the evaluation of proliferation rates displayed by the cytological samples. The CEC allowed the observation that chemotherapy induces to significant reduction in cellular proliferation. Thus, was concluded that this methodology is suitable to perform TVT diagnosis and monitoration.

ÍNDICES PROLIFERATIVOS DO TUMOR VENÉREO CANINO TRANSMISSÍVEL PELAS TÉCNICAS DO CEC E KI-67 NA CITOLOGIA ASPIRATIVA COM AGULHA FINA