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O desenvolvimento embrionario de oocitos bovinos maturados in vitro (MIV) foi avaliado em meio suplementado com liquido folicular equino (Lfe). Foram distribuidos 1045 oocitos em 11 repeticoes formando tres grupos tratamentos (T1, T2, T3) e um controle (C). O meio de maturacao utilizado foi o TCM-199 acrescido de piruvato de sodio, hormonio foliculo estimulante recombinante (rFSHh) e hormonio luteinizante equino (LHe). Suplementou-se esse meio com 10% de soro de egua em estro para o grupo controle e para T1, T2 e T3, o meio foi suplementado com 5, 10, e 20% de LFe, respectivamente. Os oocitos foram maturados in vitro (MIV) por 24h. A fecundacao in vitro (FIV) foi realizada em meio Talp-Fert. A MIV e a FIV foram realizadas em estufa a 39oC com 5% de CO2 em ar e umidade saturada. Os zigotos foram cultivados em meio SOFaaci, sob oleo mineral no interior de bolsas plasticas gaseificadas. As taxas de clivagem e de blastocistos foram observadas diariamente (D), e em D7, foram superiores (P 0,05) as do grupo controle. Em D9, a taxa de blastocistos do T2 foi superior (P 0,05). O LFe, na concentracao de 10% pode ser utilizado, em substituicao ao soro de egua em estro para suplementar o meio de MIV de oocitos bovinos. Equine follicular fluid on in vitro maturation of bovine oocytes Abstract Embryo development of bovine oocytes was evaluated using maturation medium supplemented with equine follicular fluid (eFF). One thousand and forty five (1045) oocytes were distributed in 11 replications forming three treatment groups (T1, T2 e T3) and one Control (C). TCM-199 added with sodium pyruvate, rFSHh and LHe was used as maturation medium. This medium was supplemented with 10% estrous mare serum for Control group, and 5, 10, and 20% eFF, respectively, for T1, T2 e T3 groups. In vitro maturation (IVM) of all groups was performed during 24h. In vitro fertilization (IVF) was performed in TALP-FERT medium. IVM and IVF were carried out in an incubator at 39oC with 5% CO2 in air and saturated humidity. Zygotes were cultured in SOFaaci medium, under mineral oil in gasified bags. Cleavage and blastocyst rates were daily observed (D), and at D7, were higher (P 0.05) for those from control group. At D9, blastocyst rate of T2 was higher (P 0.05). The eFF, at a 10% concentration, can replace the use of estrous mare serum to supplement the IVM medium of bovine oocytes.

LÍQUIDO FOLICULAR EQÜINO NA MATURAÇÃO IN VITRO DE OÓCITOS BOVINOS