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In order to protect human lives from disease, various biosensors having the potential to analyze a variety of biomolecules have beenutilized. Biosensors constitute one of the most promising ways to monitor and detect various biomolecules corresponding to diseases. Inthis study, we demonstrate that the reaction of streptavidin molecules with biotin on a gold electrode can be detected using the two-electrode method with a gold electrode and a platinum reference electrode. We also show the characteristics of the electrical currentresponse. While detecting 2-uM streptavidin molecules dissolved in phosphate buffered saline(PBS) solution, we found that an analyticalbiosensor can operate on the principle of detecting an antigen-antibody reaction event of protein molecules using the two-electrodemethod. We think that the “potential step” method might be useful to detect the occurrence of any antigen-antibody reactions and can becombined with other devices or ICs such as BJTs, MOSFETs, and OP-amps for the detection of biomolecules of diseases.

Detection of Protein Molecules by Electrical Current Response Using Two-Electrode Method