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The enzyme-linked immunoadsorbent assay (ELISA) is as specific and sensitive as the radioimmunoassay without the radioactive materials. The author developed the ELISA method for the determination of the bovine amelogenin over the range of 5500 ng, using the rabbit antibody against the protein. It was found that the antibody cross-reacted with the bovine enamelin only weakly and also with the porcine, murine and rat enamel proteins. The proteins in the other tissues or fluids did not cross-react with the antibody. Utilizing this ELISA procedure, the increase and decrease in the amount of enamel protein were determined during the process of the development of the murine molar germs.

Determination of mammalian enamel proteins by enzyme-linked immunoadsorbent assay using antibody against bovine enamel protein.