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This study analyzed the in vitro physiological activity of Aconitum pseudolaeve var erectum Nakai (AP) and its effect on perfume on an electroencephalogram (EEG). The results indicated that the absolute alpha power spectrum (AA) and the absolute theta power spectrum (AT) decreased significantly during more than before the inhalation of the AP perfume and its reconstruction perfume. Although there were a little different pattern in the induced part of the wave generated by the inhalation of the AP perfume and its reconstruction perfume, alpha- and theta- were shown equally. In addition, there was the same pattern in which the rate of increase reduced. The results suggest that the perfume of the AP and its reconstruction perfume have a stimulating effect on the brain. In terms of the physiological activity of AP, the activity of AP ethanol extract was significantly higher than that of water extract in DPPH, collagenase, and nitric oxide, except for an astringent effect. The AP ethanol extract was about 80% at 500 ppm in collagenase inhibition activity. In addition, the AP water and ethanol extracts were 50% at 100 ppm in the NO inhibition activity. Based on these results, we conclude that this natural substance could be used in cosmetics and in the development of perfumes.

Physiological Activity of Aconitum pseudolaeve var erectum Nakai and Its Effect on Perfume on an Electroencephalogram (EEG)