English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Objective: Tissue fixation is the most important step in morphologic preservation. While pro-tein preservation is essential in histopathology and immunohistochemistry, nucleic acid pres-ervation is important for molecular techniques. In daily practice, 10% formalin is prepared from 3740% formaldehyde stock solution. Meanwhile, the pH of the fixative needs to be kept between 6-8. In this study, the effect of variable concentrations and pH of formalin on DNA preservation was investigated with polymerase chain reaction (PCR) and fluorescein in situ hybridisation (FISH). Material and Methods: Samples with a 3-milimeter diameter were obtained from colon, thyroid and breast after resection. Samples were fixed in ten different formalin solutions prepared in variable concentrations and pH values. Genomic DNA was extracted from paraffine blocks, and the quantity and purity of the DNA were analyzed. To test the reliability of DNA fragments, PCR amplification was carried out using human β-globin gene (HBG). FISH method was applied to search the effects of varying formalin fixa-tives on hybridization kinetics using her-2/neu-17CEP dual fluorescein probes. Results: DNA was successfully extracted from all 30 samples of three different tissues fixed in 10 different concentrations and/or pH values of formalin solution. However, alterations in the formalin concentration and the pH value negatively affected FISH quality. However, alterations in pH was more effective on FISH results. Conclusion: In conclusion, during preparation and usage, adverse changes in pH and/or concentration of formalin do not affect DNA preservation sig-nificantly. However, changes in both pH and concentration of the formalin fixative may widely alter hybridization properties, so as FISH results.

The Effects of Suboptimal Formalin Fixation on DNA Content of Formalin Fixed Paraffin Embedded Tissues