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ABS TRACT Ob jec ti ve: Oxi da ti ve stress has be en imp li ca ted in the pat ho ge ne sis of ma le in fer ti lity. In this study, we ai med to me a su re the an ti o xi dant ca pa city in se mi nal plas ma in fer ti le and in fer ti le men using a re cently de ve lo ped au to ma ted me a su re ment met hod. Ma te ri al and Met hods: The study gro up inc lu ded samp les of 55 ma les from in fer ti le co up les, and 16 he althy vo lun te ers ser ved as the con trol gro up. All samp les had nor mal sperm pa ra me ters. Se mi nal to tal an ti o xi dant res pon se (TAR) and to tal oxi dant sta tus (TOS) we re de ter mi ned using co lo ri met ric met hods. Ox i da ti ve stress in dex (OSI) was cal cu la ted as [(TOS/TAR) ×100]. Fre e sulphydryl (SH) gro ups of se men samp les we re me a su red spec trop ho to met ri cally. Re sults: Se mi nal TAR was sig ni fi cantly lo wer and se mi nal TOS, OSI and SH ac ti vi ti es we re sig ni fi cantly hig her in in fer ti le gro up as com pa red with fer ti le do nors. The re we re po si ti ve cor re la ti ons bet we en TAR and sperm pa ra me ters such as +4 mo ti lity (r= 0.386, p< 0.01), and morp ho logy (r= 0.383, p< 0.01), whe re as sperm con cen tra ti ons (r= -0.333, p< 0.01) cor re la ted ne ga ti vely with TAR. In ad di ti on, TOS and OSI were, negatively cor related with sperm pa ra me ters such as mo ti lity, +4 mo ti lity and morp ho logy (p< 0.01, for all). Conc lu si on: In cre a sed oxi dant sta tus and dec re a sed an ti o xi dant res pon se and the cor re la ti on bet we en the se pa ra me ters and sperm func ti ons imp li ca ted that oxi da ti ve stress might play an im por tant ro le in ma le in fer ti lity. We fo und dec re a sed to tal an ti o xi dant res pon se in in fer ti le pa ti ents using a simp le, ra pid and re li ab le au to ma ted co lo ri met ric as say, which may su i tab le for use in any ro u ti ne an dro logy la bo ra tory.

The Evaluation of Total Antioxidant and Oxidant Response in Seminal Plasma in Fertile and Infertile Men Using a Novel Automated Method