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ABS TRACT Objective: In this study, we ha ve ai med to in ves ti ga te the po ten ti al ro le of au to lo go us bo ne mar row cell in jec ti on and mus cu lar elec tri cal sti mu la ti on as a se pa ra te and con co mi tant app li ca ti on on bo ne he a ling in ex pe ri men tal rab bit ul nar seg men tal bo ne de fect mo del. Ma te ri al and Met hods: Forty New-Ze a land rab bits, all over three months of age and we ig hing bet we en 2500 and 3500 grams we re di vi ded in to fo ur gro ups. Fo ur gro ups of rab bits we re the con trol gro up (I), electri cal sti mu la ti on gro up (II), bo ne mar row cells in jec ti on gro up (II I) and bo ne mar row cells in jec ti on with elec tri cal sti mu la ti on gro up (IV). Bo ne de fect he a ling was eva lu a ted ra di o lo gi cally ac cor ding to the mo di fi ed La ne and Sand hu sco ring system and at the end of the sixth we ek, rab bits we re sacri fi ced and the ir fo re arms we re samp led for his to pat ho lo gi cal in ves ti ga ti on. Results: When one-toone com pa ri son bet we en all gro ups was per for med, de fect he a ling was fo und to be bet ter in Gro ups II, II I, and IV com pa red to Gro up I ba sed on the ra di o lo gi cal and his to pat ho lo gi cal pa ra me ters eva lu a ted. This eva lu a ti on re ve a led that the he a ling was bet ter in gro ups tre a ted with bo ne mar row cell in jec ti on with or wit ho ut elec tri cal sti mu la ti on as well as in gro up tre a ted with elec tri cal sti mu la ti on com pa red to the con trol gro up with no tre at ment. Conc lu si on: Au to lo go us bo ne mar row cells with or wit ho ut elec tri cal sti mu la ti on, wo uld be used in he a ling of seg men tal bo ne de fect with an ade qu a te ef fi cacy. Fu tu re stem cell stu di es com bi ned with elec tri cal cur rent are re qu i red to de mons tra te and to con firm that elec tri cal cur rent en han ces in vi vo cel lu lar dif fe ren ti a ti on.

Experimental Repair of Rabbit Segmental Bone Defects by Using Autologous Bone Marrow and Electrical Stimulation