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The epidermis builds up from a lonely layer of ancestor cells. The basal layer cells directly made touch with the basement membrane, which involves reproducing cells. The keratinocyte cells of the basal layer gaining a system of intermediate filaments. In the epidermis of skin, stem cells characterised from differentiated keratinocytes according to their levitating expression of nestin. The present study investigated the expression of nestin, a recognized indicator of epidermal stem cells in ordinary human skin. Skin keratinocytes have confirmed useful, because of their fitting and ability to be cultured. The make use of cultured keratinocytes licenese a much greater surface to be covered and needs a tiny area of unaltered skin from which the skin cells were harvested for culture

EXPRESSION OF STEM CELL MARKER, NESTIN, IN THE BASAL EPITHELIAL LAYER OF SKIN EPIDERMIS BY MEANS OF IMMUNOHISTOCHEMICAL TECHNIQUE