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Isolation, identification and bioactivity test of nonpolar compunds has been conducted on n-hexaneextract sponge Haliclona (Reniera) fascigera of the island Samalona, Makassar, South Sulawesi.Isolation techniques consist of maceration, partition, fractionation using 2 steps using vacuum columnchromatography and flash column chromatography and also purification step. Identification of isolatewas analyzed with TLC, FTIR, GC-MS, and 1H NMR. The research result was obtained isolate asmuch as 4 mg and clear solid form. FTIR analysis indicate functional group OH, CH2, CH3, C=O,=CH and C-O. Identification of spot TLC under UV light and spotting solvent show that the spot wasactive on UV long and short waves and dark brown with cerium sulphate. The results show thatisolate A contain aliphatic and alcohol compounds. Bioactivity test results indicate that these isolate Abe able to inhibit the growth of bacteria Staphylococus aureus with inhibition zone diameter were11.60 mm (100 ppm); 10.40 mm (50 ppm); 9.50 mm (25 ppm); 6.50 mm (10 ppm); 6,60 mm(negative control); 17.40 mm (positive control) and against bacteria Escherichia coli with inhibitionzone diameter were 6.70 mm (100 ppm); 6,10 mm (50 ppm); 5,90 mm (25 ppm); 5.85 mm (10 ppm);5,20 mm (negative control); 18.60 mm (positive control)

zenodo (cern european organization for nuclear research)

ISOLATION, IDENTIFICATION AND BIOACTIVITY TEST OF NON POLAR COMPOUNDS ON n-HEXANE EXTRACT OF Haliclona (Reniera) fascigera FROM SAMALONA ISLAND-SPERMONDE ARCHIPELAGO