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. Bird cherry Padus avium Mill, Rosaceae, iswidespread in Ukraine, especially in forests and foreststeppeareas. Bird cherry fruits have long been used inmedicine and is a valuable medicinal raw materials. Theystated to posess astringent, anti-inflammatory, phytoncidalproperties. Bird cherry fruits are included in the USSRPharmacopoeia IX ed., The State Pharmacopoeia of theRussian Federation, The State Pharmacopoeia of Republicof Belarus. In Ukraine there are no contemporary normativedocuments for this medicinal plant material, therefore it isthe actual to develop projects in the national monographs"dry bird cherry fruit" and "fresh bird cherry fruit" to beincluded in the State Pharmacopoeia of Ukraine. Accordingto European Pharmacopoeia recommendation method ofthin-layer chromatography (TLC) is prescribed only for theidentification of the herbal drug. The principles of thinlayerchromatography and application of the technique inpharmaceutical analysis are described in StatePharmacopoeia of Ukraine. As it is effective and easy toperform, and the equipment required is inexpensive, thetechnique is frequently used for evaluating medicinal plantmaterials and their preparations. The TLC is aimed atelucidating the chromatogram of the drug with respect toselected reference compounds that are described forinclusion as reagents. Aim of this study was to developmethods of qualitative analysis of bird cherry fruits for amonograph in the State Pharmacopoeia of Ukraine (SPU).Materials and Methods. The object of our study was driedbird cherry fruits (7 samples) and fresh bird cherry fruits (7samples) harvested in 2013-2015 in Kharkiv, Poltava,Luhansk, Sumy, Lviv, Mykolaiv regions and the cityMariupol. Samples were registered in the department ofSPU State Enterprise "Pharmacopeia center". In accordancewith the Ph. Eur. and SPU requirements in "identificationC" determination was performed by TLC. TLC wasperformed on glass-backed silica gel F254Merck plates, size20x10 cm. Test solutions from samples of dried rawmaterial were prepared by extraction of 1% solution ofhydrochloric acid in 95% ethanol with ultrasound for 60minutes at 50º C. As mobile phase solvent mixture ethylacetate - anhydrous acetic acid - formic acid - water ratio(100: 10: 10: 25) was selected. For preparation thereference solution 1 mg chrysanthemin was dissolved in 10mL of 1% solution of hydrochloric acid in 95% ethanol.Fresh bird cherry fruits test solutions of the samples werecrushed to mash (free of seeds), 10 mL of methanol wasadded and treated with ultrasound for 15 minutes. Asmobile phase solvent mixture formic acid anhydrous-waterbutanolratio (16:19:65) was selected. Two referencesolutions were used. First was prepared from 2 mgchrysanthemum dissolved in 5 mL of methanol and secondwas Pharmacopoeial Reference Standard SPU of birdcherry extract dissolved in 0.2 mL of methanol withultrasound bath. Test solutions and the reference solutionswere placed onto the chromatographic plates and placedinto the chamber. When the chromatograms developed, theplates were taken out, then observed the spots in daylight.Results and discussion. In the chromatogram it wasobserved for all dry bird cherry fruits samples two pink-redcolor zones, one of which coincided for Rf and color ofchrysanthemin, and the other one was slightly lower. Thechromatogram for all samples of fresh raw material showedtwo pinkish-violet zones, one of which had Rf and colorcorresponded chrysanthemin, and the second one wasslightly lower. Compared with bird cherry extract, it wasobserved two pinkish-violet zones, coincided with Rf andcolor of bird cherry (SPU) extract zones. In accordancewith the requirements of Ph. Eur. identification by TLC isobligatory. Division of anthocyanins of plant raw materialswas observed sufficiently in our chosen solvent systemsand methods of analysis that allows to recommend thesesystems and methods of analysis for inclusion in therelevant sections of monographs for bird cherry fruit, driedand fresh for SPU. Conclusion. It was confirmed theopportunity to include in the national monographs "Birdcherry fruits fresh " and " Bird cherry fruits dry" in section"Identification C" analysis of anthocyanin by TLC inchosen systems and methods

DEVELOPMENT OF METHOD OF QUALITATIVE ANALYSIS OF BIRD CHERRY FRUIT FOR INCLUSION IN THE MONOGRAPH OF STATE PHARMACOPOEIA OF UKRAINE