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Gingival inflammations relating to an increase in the quantity of dental plaque are most frequently seen in children and young adults. Their spread and severity increase with age and reach their peak during puberty. Aim: To study the main microorganisms of the subgingival microflora in children in puberty (10 – 14 years of age). Material and methods: 60 children aged 10-14 years subjected to monitoring30 without gingivitis (up to 25% PBI) and good oral hygiene and 30 children with plaque-induced gingivitis (over 50% PBI). A PCR – RealTime method was used for identifying the main subgingival microorganisms and determining their quantities. Samples were taken with paper pins from the gingival sulcus of six teeth – three molars, two canines and one incisor (16, 13, 11, 26, 36, 43). Nine control strains were studied (a comprehensive sample). Samples were sent for research in standardized containers. Results: The results of this study show that the total quantity of subgingival microorganisms increases in the case of worsening oral hygiene and an increase in the quantity of dental biofilm. Six of the nine subgingival microorganisms tested were encountered in all children between the ages of 10 and 14. The remaining three species of microorganisms were isolated only from the children with gingivitis. Conclusion: During gingival inflammations, the subgingival microflora becomes more complex and from it can be isolated microorganisms from the red complex (P. gingivalis, T. denticola, T. forsythia).

TUDYING SUBGINGIVAL MICROORGANISMS IN CHILDREN WITH GINGIVITIS IN PUBERTY