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The dietary intake of biotin, niacin and vitamin B6 was estimated by the total diet method on the basis of the food classification and data on the apparent per capita food consumption in the Tokyo Metropolitan area obtained from the Japanese National Nutrition Survey of 1999. These vitamins were analyzed by a microbiological assay.The respective dietary intake of biotin, niacin and vitamin B6 was 45.1μg/day, 16.0mg/day and 1.26mg/day, these figures exceeding the recommended dietary allowance for adult Japanese females that was established in 2000. The main sources of biotin were meat and eggs, and constituted 30% of the dietary intake.

Estimate of the Daily Dietary Intake of Biotin, Vitamin B6 and Niacin from the 1999 Tokyo Total Diet Study