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This study was undertaken to clarify the effects of difference in sex, feeding condition and growing stage on the concentration and pattern of free amino acids in the liver. The results were as follows: 1. Although female rats gave about 20% lower values for the concentrations of both essential and nonessential free amino acids in the liver compared with those of male rats, their percentage patterns were similar to each other. The concentrations of Glu, Tyr, Val, Ileu, Leu and Phe, were particularly lowered in female rats. 2. In rats receiving a protein free diet, the concentrations of Lys and His were elevated, but those of Val, Ileu, Leu and Phe were lowered compared with these values in rats receiving a standard diet. On the other hand, only Ser among non-essential amino acids was increased. The free amino acid pattern was remarkably different in the same way between the two groups. In fasted rats, only Thr among essential amino acids and most of non-essential amino acids were much lowered. But the pattern for other amino acids was almost similar to that of the standard group. 3. Free amino acid concentration was changed in rats after growing as follows: (1) Thr and Ala were increased, (2) Ser, Glu, Gly, Cys, Met, Leu and Phe were decreased. Val, Ileu, Lys and His were almost unchanged. Jap. J. Nutr., 34 (6) 263-270 (1976)

On the Concentration of Free Amino Acids in Liver of Rats Different in Sex, Feeding Condition and Growing Stage