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Measurement of carbon dioxide (CO2), oxygen (O2), and nitrogen (N2) concentration in modified atmosphere packaging (MAP) food is critical to be carried out by the food industry. A slight variation in concentrations of CO2, O2, and N2 in food packaging may have a significant impact on product quality and safety for human health. Accurate and reliable measurement of CO2, O2, and N2 concentrations in food packaging is crucial, and it can only be achieved by calibrating the gas analyzer. This study aimed to develop gas mixtures for the calibration of CO2, O2, and N2 gas analyzers at a typical concentration range of modified atmosphere packaging. The calibration gas mixtures were prepared gravimetrically by following ISO 6142. The concentration ranges of CO2, O2, and N2 for calibration gas mixtures were set at 9-19% mol/mol, 1-5% mol/mol, and 74-88%mol/mol, respectively. Each parent gas was identified for its impurities using gas chromatography with a pulsed discharge helium ionization detector (GC-PDHID). The compositions of CO2, O2, and N2 in the mixtures were verified by evaluating the internal consistency within the prepared gas mixtures using gas chromatography with a thermal conductivity detector (GC-TCD). The short term stability of the prepared gas mixtures was evaluated using an equal division method. The result showed that good internal consistency was achieved between the gravimetrical and GC’s verification values, having linear regression coefficient (R2) ≥ 0.999. The t-test result has shown that CO2 has better short term stability than O2 and N2. In conclusion, the developed calibration gas mixtures at a typical concentration range of modified atmosphere packaging have shown satisfying results for CO2 component. However, further evaluation is still required to minimize the instability of O2 and N2 components.

periódico tchê química

DEVELOPMENT OF CALIBRATION GAS MIXTURES (CARBON DIOXIDE AND OXYGEN IN NITROGEN MATRIX) AT A TYPICAL CONCENTRATION RANGE OF MODIFIED ATMOSPHERE PACKAGING