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Department of chemistry, Dankook University, Anseo-dong, Cheonan 330-714, Republic of Korea(Received January 25, 2011 : Accepted February 21, 2011)Abstract: This paper describes an electrochemical immunosensor for simple, fast and quanti-tative detection of a urinary hippuric acid which is one of major biological indicator in toluene-exposed humans. The feature of this electrochemical system for immunoassay of hippuric acidis based on the direct conjugation of ferrocene to a hippuric acid. With the competition betweenthe ferrocene-hippuric acid complex and hippuric acid for binding to the anti-hippuric acidmonoclonal antibody coated onto gold nanoparticles, the electrical signals are turned out to beproportional to urinary hippuric acid in the range of 0.01-10 mg/mL, which is enough to beused for the point-of-care. The proposed electrochemical method could extend its applicationsto detect a wide range of different small molecules of antigens in the health care area.Keywords : Immunosensor, Hippuric acid, Ferrocene, Point-of-care

Simple Electrochemical Immunosensor for the Detection of Hippuric Acid on the Screen-printed Carbon Electrode Modified Gold Nanoparticles