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Objective: The aim of this study was to evaluate the detection of Legionella species from the respiratory tract samples by Polymerase Chain Reaction method. Methods: The bronchoalveolar lavage fluid and transtracheal aspiration samples of patients with atypical pneumonia hospitalized in the Intensive Care Unit of Chest Diseases Department were examined to identify Legionella species by Polymerase Chain Reaction method. This method was performed to amplify a 386-bp product within the 16SrRNA gene of Legionella pneumophila in these samples sent to Bacteriology Laboratory of this department. Results: The Polymerase Chain Reaction method was applied to 56 respiratory samples and only in one specimen Legionella species was identified. Conclusion: It was concluded that for patients with atypical pneumonia Polymerase Chain Reaction method is fast and effective to isolate Legionella species from the respiratory samples.

Detection of Legionella species from bronchoalveolar lavage fluids and transtracheal aspiration samples by Polymerase Chain Reaction method