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To explore the influence of the composite enzymes on harmful components in reconstituted tobacco, we developed compound enzymes which included power of cellulose (23-41%, 100,000U/g), xylanase (10-23%, 100,000U/g), complex protease (41-57%, 100,000U/g), pectinase (5-6%, 100,000U/g) and amylase (1.5-3.8%, 10,000U/g). The compound enzymes were added to the reconstituted tobacco (0.4% enzymes, incubated for 1 h at 45 ± 5°C). We evaluated the quality of the reconstituted tobacco using physical and chemical indices. The results show that protein and pectin contents were effectively reduced, with improved taste of the cigarette smoke, and harmfu l ingredients were reduced, such as ammonia and hydrogen cyanide, in the mainstream smoke of the composite enzyme treatment group compared with the untreated control group. This approach will reduce the danger of mainstream smoke in reconstituted tobacco.

Effect of Enzyme on Harmful Components Reduction in Reconstituted Tobacco