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We have developed a lab prototype of a microfluidic flow analyzer, which is capable of quick and efficient analysis of biological samples. Low cost and portability makes it suitable for point of care diagnostics in rural area of developing countries. A significant size reduction has been achieved by choosing a microfluidic flow and pumping system, micro-electronic components, integrated circuits boards, and fiber optics. A two dimensional microfluidic chip fabricated with nanolithography technique integrates the fluidics and optics into a single platform. Forward scatters (FSC), side scatter (SSC) and fluorescence (FL) are measured from polystyrene beads as well as from different live cells. Overall dimension achieved for the final prototype is 39 cm x 22 cm x 10 cm.

Development of a Lab on a Chip Flow Cytometer - Portable and Affordable Flowcytometer for Point of Care Diagnostics in Rural Areas