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This project focuses on the development of an acoustic driven, freely programmable multifunctional biochemical lab-on-a-chip. By combining different platform elements, like microdissection-, nanofluidicand detection-modules, the lab-on-a-chip can be adapted to questionand patient-specific cytogenetic and forensic applications. In contrast to many common lab-on-a-chip approaches presently available, the fluidic handling is done on a planar surface of the lab-on-a-chip. Minute amounts of biochemical fluids are confined in ‘virtual’ reaction chambers and ‘virtual’ test tubes in the form of free droplets. The droplets, fluidic tracks and reaction sites are defined at the chip surface by a monolayer chemical modification of the chip surface. Surface acoustic waves are employed to agitate and actuate these little ‘virtual’ test tubes along predetermined trajectories. Well-defined investigations, controlled in the submicrolitre regime, can be conducted quickly and gently on the lab-on-a-chip.

PROGRAMMABLE CYTOGENETIC SUBMICROLITRE LAB-ON-A-CHIP FOR MOLECULAR DIAGNOSTIC APPLICATIONS