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Today, identification of pathogenic bacteria using modern and\udaccurate methods is inevitable. Integration in electrochemical measurements\udwith nanotechnology has led to the design of efficient and sensitive DNA\udbiosensors against bacterial agents. Here, efforts were made to detect\udAeromonas hydrophila using aptamers as probes and zinc sulfide (ZnS)\udnanospheres as signal enhancers and electron transfer facilitators. After\udmodification of the working electrode area (in a screen-printed electrode)\udwith ZnS nanospheres through electrodeposition, the coated surface of a\udmodified electrode with ZnS nanospheres was investigated through scanning\udelectron microscopy (SEM). The size of synthesized ZnS nanospheres was\udestimated at about 20–50 nm and their shape was in the form of porous\udplates in microscopic observations. All electrochemical measurements were\udperformed using cyclic voltammetry (CV), electrochemical impedance\udspectroscopy (EIS), and constant potential amperometry (CPA) techniques. The\uddesigned DNA biosensor was able to detect deoxyribonucleic acid (DNA) of\udAeromonas hydrophila in the range 1.0  ×  10−4 to\ud1.0  ×  10−9 mol L−1; the limit of detection (LOD) in this\udstudy was 1  ×  10−13 mol L−1. This DNA biosensor showed\udsatisfactory thermal and pH stability. Reproducibility for this DNA biosensor\udwas measured and the relative standard deviation (RSD) of the performance of\udthis DNA biosensor was calculated as 5 % during 42 days

A DNA biosensor for molecular diagnosis of &lt;i&gt;Aeromonas hydrophila&lt;/i&gt; using zinc sulfide nanospheres

A DNA biosensor for molecular diagnosis of <i>Aeromonas hydrophila</i> using zinc sulfide nanospheres