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Fluorescence analysis, being a non-invasive technique, has become one of the most powerful and widely used techniques formicrobiologists and chemists to study various types of sample from photosynthetic microbes to hydrocarbons. The work reportedhere focuses on experimental results of fluorescent features of photosynthetic microbial species (cyanobacteria) and also fivedifferent crude oil samples. The cyanobacteria samples were collected from the Baltic Sea at the end of July 2011 and wereassociated with cyanobacterial bloom events, and the crude oil samples were from various oil spill events. The aim of the study wasto find fluorescent biosignatures of cyanobacteria (initially a species specific to the Baltic Sea) and the fingerprints of crude oil; oilspills can be difficult to differentiate from biogenic films when using Synthetic Aperture Radar (SAR) or sunglint contaminatedoptical imagery. All samples were measured using a Perkin Elmer LS55 Luminescence spectrometer over a broad range of excitationand emission wavelength from ultraviolet (UV) to near infrared (NIR). The results are presented in Excitation Emission Matrices(EEMs) that exhibit the fluorescent features of each sample. In the EEM of the seawater sample containing cyanobacteria, there is anintense emission peak from tryptophan with fluorescent excitation and emission peaks at 285 and 345 nm respectively. In addition,fluorescent signatures of phycocyanin and chlorophyll-a are present with excitation and emission centre wavelengths at 555 nm, 645nm and 390 nm, 685 nm, respectively. Additionally, the fluorescence signatures of Polycyclic Aromatic Hydrocarbons (PAHs) arepresent in the EEMs of crude oil samples with excitation and emission peaks at 285 nm and 425 nm. This study underpins furtherresearch on how to distinguish cyanobacteria species by their fluorescence signatures and the potential role that PAHs play indetection of cyanobacteria fluorescence features

the international archives of the photogrammetry, remote sensing and spatial information sciences/international archives of the photogrammetry, remote sensing and spatial information sciences

FLUORESCENT ANALYSIS OF PHOTOSYNTHETIC MICROBES AND POLYCYCLIC AROMATIC HYDROCARBONS LINKED TO OPTICAL REMOTE SENSING