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As research into the biotic effects of ocean acidification hasincreased, the methods for simulating these environmental changes in thelaboratory have multiplied. Here we describe the atmospheric carbon controlsimulator (ACCS) for the maintenance of plankton under controlled <i>p</i>CO<sub>2</sub>conditions, designed for species sensitive to the physical disturbanceintroduced by the bubbling of cultures and for studies involving trophicinteraction. The system consists of gas mixing and equilibration componentscoupled with large-volume atmospheric simulation chambers. These chambersallow gas exchange to counteract the changes in carbonate chemistry inducedby the metabolic activity of the organisms. The system is relatively lowcost, very flexible, and when used in conjunction with semi-continuousculture methods, it increases the density of organisms kept under realisticconditions, increases the allowable time interval between dilutions, and/ordecreases the metabolically driven change in carbonate chemistry during theseintervals. It accommodates a large number of culture vessels, whichfacilitate multi-trophic level studies and allow the tracking of variableresponses within and across plankton populations to ocean acidification. Italso includes components that increase the reliability of gas mixing systemsusing mass flow controllers

biogeosciences

Technical Note: A minimally invasive experimental system for &amp;lt;i&amp;gt;p&amp;lt;/i&amp;gt;CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator)